Article 8: ANTIFUNGAL POTENTIAL OF ACTINOMYCETES ISOLATED FROM FIVE DIFFERENT SOURCES AGAINST Aspergillus flavus AND Fusarium verticillioides (Vol.1, Iss. 2; pp-180-186)

Ologuntuyi O. O*1 and AGBOOLA, S. A2

Text | Abstract | PDF

ANTIFUNGAL POTENTIAL OF ACTINOMYCETES ISOLATED FROM FIVE DIFFERENT SOURCES AGAINST Aspergillus flavus AND Fusarium verticillioides 

Ologuntuyi O. O*1 and AGBOOLA, S. A2

  1. Department of Microbiology, Adekunle Ajasin University Akungba-Akoko, Ondo State, Nigeria.
  2. Department of Microbiology, Federal university of technology Akure, Ondo State. Nigeria

*Corresponding Author’s email address: haryurbhunmmy@gmail.com;Tel: +2347035647924

 

A R T I C L E  I N F O

Article Type: Research

Received: 01, Nov. 2018.

Accepted: 31, Dec. 2018.

Published: 31, Dec. 2018.

 

 

 A B S T R A C T

This research work is to investigate the antifungal potential of actinomycetes isolated from five different sources against Aspergillus flavus and Fusarium verticillioides. Isolation of microorganisms from the soil samples was carried out by serial dilution and pour plate techniques. The soil isolates were screened for their antagonistic ability against mycotoxigenic fungi: A. flavus and F. verticillioides. The isolates include Actinomyces spp, Mycobacterium spp, Rhodococcus spp, Nocardia spp, Streptomyces spp, and Streptomonospora spp. The results indicate that all the bacteria species exhibited varying degrees of biological control potential against the tested fungi. Out of all the actinomycetes use, Streptomyces spp appeared to have the highest diameter zone of inhibition against A. flavus of 16.00 mm and against F. verticillioides of 21.00 mm. The results showed the antimicrobial activity of the actinomycetes species, and thus indicate the potential of using them as bio-fungicidal agents in reducing the damages and threat caused by A. flavus and F. verticillioides in crop production.
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References 
1. Braga, R. M., Dourado, M. N. and Araújo, W. L. (2016). Microbial interactions: ecology in a molecular perspective. Brazillian Journal of Microbiology, 47(1): 86-98.
2. Cheesbrough, M. (2006). District Laboratory Practice for Tropical countries Countries, part 2, second Edition and Cambridge University Press. Cambridge.
3. Egbuta, M. A., Mwanza, M. and Babalola, O. O. (2017). Health Risks Associated with Exposure to Filamentous Fungi. International Journal of Environmental Research Public Health. 14(7):719.
4. Gohel, V., Singh, A., Vimal, M., Ashwini, P. and Chhatpar, H. S. (2006). Review-Bioprospecting and antifungal potential of chitinolytic microorganisms. African Journal of Biotechnology. 5(2): 54–72.
5. Hoell, I. A., Dalhus, B., Heggset, E. B., Aspmo, S. I. and Eijsink, V. G. (2006). Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes. FEBS Journal 273(21):4889–900.
6. Kamara, V. and Gangwar, M. (2015). Antifungal Activity of actinomycetes from rhizospheric soil of medicinal plants against phytopathogenic fungi. Intrenational Journal of Current Microbiology and Applied Science. 4(3): 182-187.
7. Loi, M., Fanelli, F., Liuzzi, V. C., Logrieco, A. F. and Mulè, G. (2017). Mycotoxin Biotransformation by Native and Commercial Enzymes: Present and Future Perspectives. Toxins (Basel).9(4): 111.
8. Matsumoto, K. S,, Guevara-Gonzalez, R. G. and Torres-Pacheco, I. (2006). Fungal chitinases. advances in agricultural and food biotechnology. New York: Elsevier; pp 289–304.
9. Njenga, W. P., Mwaura, F. B. and Wagacha, J. M. (2017) Methods of Isolating Actinomycetes from the Soils of Menengai Crater in Kenya. Arch Clinical Microbiology. 8:3.
10. Nyongesa, B.W., Okoth, S. and Ayugi, V. (2015) Identification Key for Aspergillus Species Isolated from Maize and Soil of Nandi County, Kenya. Advances in Microbiology, 5, 205-229.
11. Rahman, A. M., Mohammad, Z. I. and Md. Anwar Ul I. (2011). Antibacterial Activities of Actinomycete Isolates Collected from Soils of Rajshahi, Bangladesh, Biotechnology Research International, pp 6-9.
12. Sobia, M., Amna, A., Ibatsam, K. and Irum, M. (2010). Antagonistic potential of soil bacteria against food borne fungi. World Applied Sciences Journal. 11(8): 966-969.
13. Torsvik, V. And Ovreas, L. (2002) Microbial diversity and function in soil: from genes to ecosystems. Current Opinion in Microbiology, 5: 240 – 245.
14. Wohlleben, W., Mast, Y., Stegmann, E. and Ziemert, N. (2016). Antibiotic drug discovery. Microbial Biotechnology. 9(5):541-8