BACTERIOLOGICAL QUALITY OF COMMONLY VENDED STREET FOODS AND ANTIMICROBIAL RESISTANCE PATTERNS OF THE BACTERIAL ISOLATES
BACTERIOLOGICAL QUALITY OF COMMONLY VENDED STREET FOODS AND ANTIMICROBIAL RESISTANCE PATTERNS OF THE BACTERIAL ISOLATES
Md. Rashed Sarkar[1], Md. Fakhruzzaman[2], Farzana Afroz[3], and Md. Tazul Islam Sarkar4
*Corresponding author E-mail: fakhrul.hstu@gmail.com
| A R T I C L E I N F O
Article Type: Research Received: 05, Aug.2023. Accepted: 16, Sep. 2023. Published: 29, Oct.2023.
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A B S T R A C T
Food safety problems are particularly becoming an increasingly serious threat to public health in developing countries. This study aimed to assess the bacterial load, and isolate, identify, and characterize bacterial isolates among commonly vended street foods in Dinajpur City of Bangladesh. Antimicrobial susceptibility of isolates was also performed using commonly used antibiotics. A total of 64 street food samples from four different food items were aseptically collected, analyzed, and bacteria were counted by standard plate count method. Ten grams of each food sample was transferred in to 90 ml of buffered peptone water and homogenized. The homogenates were serially diluted and a volume of 0.1 ml dilution was spread on solid media and incubated at 35-37 °C for 24 h. The study revealed that 39 (61%) of the food samples had pathogenic bacterial contamination. Three different bacterial species including Escherichia coli (18.75%), Klebsiella spp. (6.25%) and Staphylococcus spp. (35.93%) were isolated. The Total Viable Count (TVC) in singara ranging from 2.0 to 2.9 CFU/g, in sugar cane juice ranging from 3.1 to 3.8 CFU/ml, in jilapi ranging from 2.1 to 3.6 CFU/g and in chola ranging from 2.7 to 3.5 CFU/g. The antibiotic susceptibility testing was done for isolated species using the Kirby-Bauer disk diffusion method. Antibiogram study of the isolated organisms revealed that isolated E. coli was found to be resistant to doxycycline, ampicillin, neomycin, cefixime, norfloxacin, levofloxacin, and azithromycin. Staphylococcus spp. isolates were found to be resistant to methicillin, ampicillin, amoxicillin, and penicillin. And Klebsiella spp. isolates were found to be resistant to amoxicillin, cloxacillin, cefixime, and imipenem. Ciprofloxacin was found to be the most effective antimicrobial against all isolates. This study confirmed considerable rate of contamination in street vended foods in Dinajpur City. The identified foodborne bacteria and antibiotic resistant isolates could pose a public health problem in that locality. Therefore, regular inspection, health education, and training of vendors on food handling and safety practices are recommended. |
[1] Md. Rashed Sarkar, MS Student, Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh.
[2] Md. Fakhruzzaman, Professor, Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh.
[3] Farzana Afroz, Associate Professor, Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh.
4 Md. Tazul Islam Sarkar, PhD Fellow, Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh.
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