Article 5: ULCER-HEALING ACTIVITY OF ARCHACHATINA MARGINATA MUCIN ON INDOMETHACIN-INDUCED GASTRIC ULCERATION IN ALBINO RATS (Vol. 1, Iss. 1: pp. 53-60)

Amah A.K, Anigo K.M, Owolabi O.A, Ejiofor D.C, Ewa O, Wassagwa, J

Text | Abstract | PDF

1Amah A.K, 2Anigo K.M, 2Owolabi O.A, 1Ejiofor D.C, 3Ewa O, 2Wassagwa, J

 

1Department of Human Physiology, Imo State University, Owerri Imo State.

2Department of Biochemistry, Ahmadu Bello University, Zaria, Kaduna State.

3Department of Medical Biochemistry, Gregory University Uturu Abia State.

 

Corresponding author:

Ewa Ogbonnaya*

Address: Department of Medical Biochemistry, College of Medicine, Gregory University, Zaria Nigeria.

Tel: +2348023837330, +2348107357039

E-mail: ewahalfred@gmail.com

 

A R T I C L E  I N F O

Article Type: Research

Received: 06, Sep. 2018.

Accepted: 15, Oct. 2018.

Published: 15, Oct. 2018.

 

 

 A B S T R A C T

The objective of this study was to evaluate the ulcer healing activity of Archachatina marginata mucin on indomethacin-induced gastric ulceration in albino rats. Methods: Thirty (30) adult male albino rats were divided into six groups of five rats each. Group, I was the normal control and was administered with 2ml/kg b.w distilled water. Group II was induced with 120mg/kg b.w indomethacin only. Groups III, IV and V were induced and treated with 200, 400 and 800mg/kg b.w mucin respectively while group VI was induced and treated with 20mg/kg b.w omeprazole. Treatment lasted for 10days after which animals were sacrificed by cervical dislocation. Harvested stomach tissue and gastric juice were processed and analyzed using standard methods. Result: Oral administration of indomethacin caused ulceration at the glandular region of rat stomach indicated by a high ulcer index (6.28±0.33). However, this was low in group V administered with 800mg/kg mucin (1.52±0.29). A similar value was recorded for the omeprazole treated group (1.24±0.25). Gastric mucus was reduced in the stomach tissue of rats in group II (0.22±0.05 abs/g of tissue). However, contrary values were recorded for other groups. This was obvious in groups V and VI (0.5±0.08 abs/g of tissue) and (0.53±0.07 abs/g of tissue) respectively. Free acidity and total acidity were significantly higher in group II (63.40±7.66mEq/L) and (91.40±6.34mEq/L) respectively. However, the reduction in free acidity and total acidity was observed in the group treated with 800mg/kg b.w mucin (47.40±7.19mEq/L) and (71.00±7.00mEq/L) respectively. A similar observation was made on the omeprazole treated group (34.00±5.40mEq/L) and (54.80±5.35mEq/L) respectively. Conclusion: By the outcome of this study, It can be deduced that mucin from A. marginata possesses appreciable ulcer healing potential
Keywords:

A. marginata mucin, indomethacin, omeprazole, gastric ulcer

References 

  1. Abere, S.A. and Lameed, G.A. (2008). The medicinal utilization of snails in some selected states in Nigeria. In: Proceeding of the first National Conference of the Forests and Forest Products Society of Nigeria 233 – 237.
  2. Adikwu, M.U and Ikejiuba, C.C. (2005). Some physicochemical and wound healing properties of snail mucin. Boll Chim Farm. 145:(2), 5.
  3. Adikwu, M.U. and Alozie, B. U. (2007). Application of snail mucin dispersed in detarium gum
  4. Adiukwu, M.U. (2005). Evaluation of snail mucin motif as rectal absorption enhancer for insulin in non-diabetic rat model. Biological pharmaceutical Bulletin, 28:(9), 18011-1804.
  5. Amani, S.A., Reham, M.E., Gamal, A.S. (2012). Natural products in the treatment of ulcerative colitis and Peptic ulcer. Journal of Chemical Society, 17,101-124.
  6. Cho, C.H., Ogle, C.W., Dai, S. (1976).Acute gastric ulcer formation in response to electrical vagal stimulation in rats. European Journal of Pharmaceutical Sciences, 35:215-219.
  7. Corne, S.J., Morrissey, S.M., Wood, R.J. (1974). A method for the quantitative estimation of gastric barrier mucus. London Journal of physiology, 242, 116-117.
  8. El-far, A., Gindi, R.R, AbdEl-Maksoud, H.A.A. (2012). Some biochemical changes followed experimental gastric Ulceration.World Academy of Science Engineering and Technology, 70, 1132.
  9. eOpoka, D., Adamek, D., Plonka, M., Reczynski, W., Bas, B., Drozdowicz, D., Jagielski, P., Sliwoski, Z., Adamski, P., Brzozowski, T. (2010). Importance of Luminal and Mucosal Zinc in the mechanism of experimental gastric ulcer. Journal of Physiology and Pharmacology, 5:(61), 581-591.
  10. gel in wound healing. Scientific Research and Essay., 2 (6), 195-198.
  11. Jiang, T., Tang, C.P., Chen, Y.F., Feng, Y.F., Chu, B., He, J.J., Chen, Q.Y.(2009). Officinarum flavonoids’ influence on rat gastric ulcer model. Chinese Journal of Herbal Medicine 32:(2), 261.
  12. Kaur, A., Singh, R., Sharma, R., Kumar, S. (2012). Peptic ulcer review on ethiology and pathogenesis. International Research Journal of Pharmacy, 3:(6)34.
  13. Kirchhoff, P., Thenral, S., Shafik, S., Andrew, D., Tobias, B., Christain, G., Carsten, T.V., Christoph, B.D.O., John, P.G.(2010). Zinc salts provide a novel, prolonged and rapid inhibition of gastric acid secretion. American Journal of Gastroenterology, 327:(106), 62–70.
  14. Kulkarni SK.(2010). Hand book of Experimental Pharmacology. 3rd revised and enlarged edition Delhi: Vallabh Prakashan, 149.
  15. Lindén, S.K., Sutton, P., Karlsson, N.G., Korolik, V., McGuckin, M.A.(2008). Mucins in the mucosal barrier to infection. Immunology, 1, 183–197.
  16. Lorke, D. 1983.New approach to practical acute toxicity. Archive of Toxicology, 6(13), 54.
  17. Oladapo, O.O, Abiodun, A., Olugbemiga, O., Oladipo, O., Olutosin, A.(2012). Survey of Wild animals used in Zootherapy at Ibadan Oyo State Nigeria. International journal of Molecular Zoology, 2:(9),70-73.
  18. Tarnawski, A., Szabo, I.L., Husain, S.S., Soreghan, B. (2001). Regeneration of gastric mucosa during ulcer healing is triggered by growth factors and signal transduction pathways. Journal of Physiology 95:337-44

Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 Unported License.