NEXT-GENERATION HIGH-THROUGHPUT SEQUENCING TECHNOLOGIES: CONCEPT AND APPLICATIONS
The development of DNA sequencing more than 35 years ago has profoundly impacted biological research. In the last few years, remarkable technological innovations have emerged that allow the direct and cost-effective sequencing of complex samples at unprecedented scale and speed. These next-generation technologies make it feasible to sequence not only static genomes, but also entire transcriptomes expressed under different conditions. These and other powerful applications of next-generation sequencing are rapidly revolutionizing the way genomic studies are carried out. In this article, the steps and applications of Next generation sequencing is discussed