MOLECULAR CHARACTERIZATION AND ANTIBIOGRAM STUDY OF BACTERIA ISOLATED FROM DIARRHOEIC CALVES

Nasima Yesmen Mousome, Md. Ismail Hossen, Md. Khaled Hossain, Nazmi Ara Rumi

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MOLECULAR CHARACTERIZATION AND ANTIBIOGRAM STUDY OF BACTERIA ISOLATED FROM DIARRHOEIC CALVES

Nasima Yesmen Mousome1, Md. Ismail Hossen2*, Md. Khaled Hossain3, Nazmi Ara Rumi4

1Nasima Yesmen Mousome, MS student, Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh.

2* Md. Ismail Hossen, Assistant Professor, Department of Medicine, Surgery & Obstetrics, Hajee Mohammad Danesh Science & Technology University, Dinajpur, Bangladesh.

3 Md. Khaled Hossain, Professor, Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh.

4 Nazmi Ara Rumi, Associate Professor, Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh.

*Corresponding author E-mail: ismailvet@hstu.ac.bd

 

A R T I C L E  I N F O

Article Type: Research

Received: 04 May. 2024.

Accepted: 15 July. 2024.

Published: 03 Aug. 2024.

 

 

 A B S T R A C T

This study was conducted to investigate the causative agents of bacterial infections in diarrhoeic calves and to assess their antibiotic susceptibility patterns in Dinajpur Sadar, Bangladesh. A total of forty-five (45) fecal samples were collected and examined using conventional microbiological methods, including bacterial culture, biochemical tests, and antibiotic sensitivity assays. Molecular characterization of Escherichia coli and Salmonella spp. was performed using PCR with universal primers targeting the 16S rRNA and invA gene respectively. The bacterial isolates identified from the diarrhoeic samples were E. coli (53.33%), Shigella spp. (15.5%), Salmonella spp. (20%), Enterobacter spp. (8.88%), and Bacillus spp. (2.22%). The bacterial isolates showed resistance to amoxicillin, ampicillin, erythromycin, and cephalexin. E. coli exhibited sensitivity to azithromycin, cotrimoxazole, doxycycline, and levofloxacin. Shigella spp. was sensitive to cefixime, tetracycline, and gentamycin. Enterobacter spp. showed sensitivity to azithromycin and cefixime. Salmonella spp. was sensitive to tetracycline and streptomycin. Bacillus spp. exhibited susceptibility to streptomycin and bacitracin. Continued monitoring of antimicrobial resistance in livestock is essential to guide rational antibiotic use and protect public health in Bangladesh.
KEYWORDS:

Prevalence, molecular characterization, antibiogram study, bacteria, diarrhoeic calves.

 

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