EFFECTS OF PSEUDOMONAS SYRINGAE AND XANTHOMONAS CUCURBITAE ON SEED GERMINATION OF CUCURBITS

Md. Estiak Khan Chowdhury, Md. Enamul Haque, Fahmida Begum Mina, Sumon Karmakar, Mutasim Billah, Meherun Nesa, Biswanath Sikdar and Md. Faruk Hasan

Text | Abstract | PDF

EFFECTS OF PSEUDOMONAS SYRINGAE AND XANTHOMONAS CUCURBITAE ON SEED GERMINATION OF CUCURBITS

Md. Estiak Khan Chowdhury1,2, Md. Enamul Haque3, Fahmida Begum Mina3, Sumon Karmakar3, Mutasim Billah3, Meherun Nesa4, Biswanath Sikdar3 and Md. Faruk Hasan4,*

  1. Institute of Veterinary Pathology, Justus Liebig University, FB 10-Veterinary Medicine Frankfurter Str. 96, 35392, Giessen, Germany.
  2. Department of Genetic Engineering and Biotechnology, University of Rajshahi. Rajshahi, P.O. Box 6205, Bangladesh.
  3. Department of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj, P.O. Box 8100, Dhaka, Bangladesh.
  4. Department of Zoology, University of Rajshahi, Rajshahi, P.O. Box 6205, Bangladesh.
  5. Department of Microbiology, University of Rajshahi. Rajshahi-6205, Bangladesh.

*Corresponding author e-mail: faruk_geb@ru.ac.bd       

A R T I C L E  I N F O

Article Type: Research

Received: 6, Sep. 2024.

Accepted: 10, Sep. 2024.

Published: 17, Sep. 2024.

 

 

 A B S T R A C T

Pseudomonas syringae and Xanthomonas cucurbitae affect the leaves, stems, and fruits of cucurbit plants contributing to a widespread bacterial disease of cucurbits. The present study was conducted to evaluate the germination rate of the seeds of five species of cucurbit, viz. bitter gourd, ridge gourd, bottle gourd, cucumber, and pumpkin, by inoculating bacteria P. syringae and X. cucurbitae at the seedling stage of development. The germination test was carried out by using the ‘Top-of-paper’ method. Bitter gourd seeds showed an 80% germination rate in control, but seeds inoculated with P. syringae and X. cucurbitae showed a 20% germination rate after 90 hours of incubation. A similar delay resulted for ridge gourd seeds inoculated with P. syringae and X. cucurbitae showed highest 25% and 41.67% germination, respectively. Bottle gourd seeds inoculated with P. syringae and X. cucurbitae showed a maximum of 63.64% and 72.73% germination respectively, after 120 hours. After 60 hours of incubation, cucumber seeds inoculated with P. syringae and X. cucurbitae showed 50% and 70% germination, respectively. However, after 90 hours of incubation, cucumber seeds inoculated with isolate P. syringae and X. cucurbitae showed the highest of 100% germination. Similarly, pumpkin seeds inoculated with P. syringae and X. cucurbitae showed a maximum of 70% and 80% germination after 50 hours of incubation. The experiment revealed that inoculation of Pseudomonas syringae and Xanthomonas cucurbitae at the seedling stage cucurbits have impaired seed germination significant delay in seed vigor over control.
Keywords:

Cucurbits, seed germination, bacteria inoculation, top-of-paper.

 

 

References

  1. Bicksler, A. J. (2011). Testing seed viability using simple germination tests. ECHO Asia, 452.
  2. Botlagunta, N. & Babu, S. (2024). Growth enhancement and changes in bacterial microbiome of cucumber plants exhibited by biopriming with some native bacteria. Saudi Journal of Biological Sciences. 31, 103997.
  3. Chowdhury, M.E.K., Chaity, A.S., Khan, A., Islam, M.A. Sikdar, B. & Hasan, M.F. (2020). Molecular Characterization of the Pathogen Responsible for Choanephora Fruit Rot Disease in Momordica Charantia (L.) and Establishment of Its Ecofriendly Control Measures. GSC Biological and Pharmaceutical Sciences, 11(3), 22-33.
  4. Chowdhury, M.E.K., Haque, M.E., Nesa, M., Sikdar, B., & Hasan, M.F. (2024). Identification and biological control of bacterial leaf spot disease of cucurbits. World Journal of Advanced Research and Reviews, 23(01), 2480–2491.
  5. Costa, L. E. de O., Queiroz, M. V. de, Borges, A. C., Moraes, C. A. de, & Araújo, E. F. de. (2012). Isolation and characterization of endophytic bacteria isolated from the leaves of the common bean (Phaseolus vulgaris). Brazilian Journal of Microbiology, 43(4), 1562–1575.
  6. Enroth, C. (2020). The Many Different Types of Cucurbits. College of Agricultural, Consumer & Environmental Sciences,
  7. Finch-Savage, W. E., & Bassel, G. W. (2016). Seed vigour and crop establishment: extending performance beyond adaptation. Journal of Experimental Botany, 67(3), 567–591.
  8. Gaber, S. D., & Roberts, E. H. (1969). Water sensitivity in barley seeds II. association with micro-organism activity. Journal of the Institute of Brewing, 75(3), 303–314.
  9. Garcia, D., Zhao, S., Arif, s., Zhao, Y., Ming, L.C., & Huang, D. (2022). Seed Priming Technology as a Key Strategy to Increase Crop Plant Production under Adverse Environmental Conditions. J Agri Horti Res., 5(1), 27-46.
  10. Gómez-Favela, M. A., Gutiérrez-Dorado, R., Cuevas-Rodríguez, E. O., Canizalez-Román, V. A., del Rosario León-Sicairos, C., Milán-Carrillo, J., & Reyes-Moreno, C. (2017). Improvement of chia seeds with antioxidant activity, GABA, essential amino acids, and dietary fiber by controlled germination bioprocess. Plant Foods for Human Nutrition, 72(4), 345–352.
  11. Gouda, S., Kerry, R. G., Das, G., Paramithiotis, S., Shin, H.-S., & Patra, J. K. (2018). Revitalization of plant growth promoting rhizobacteria for sustainable development in agriculture. Microbiological Research, 206, 131–140.
  12. Griffin, D. M. (1966). Fungi attacking seeds in dry seed-beds. Linn. Soc. NSW, 91, 84–89.
  13. Harper, S. H. T., & Lynch, J. M. (1979). Effects of Azotobacter chroococcum on barley seed germination and seedling development. Microbiology, 112(1), 45–51.
  14. Harper, S. H. T., & Lynch, J. M. (1981). Effects of fungi on barley seed germination. Microbiology, 122(1), 55–60.
  15. Hussain, T., Ishtiaq, M., Azam, S., Maqbool, M., & Mushtaq, W. (2017). Investigation of Seed Damaging Pathogens Associated with Wheat Crop in Bhimber Azad Kashmir, Pakistan and Their Managements. Journal of the Chemical Society of Pakistan, 39(1), 161–168.
  16. Khaskheli, M. I., Jiskani, M. M., Nizamani, I. A., Khaskheli, A. J., Chang, X., & Anum, A. (2019). Association of seed mycoflora with peas Pisum sativa seeds. International Journal of Environment, Agriculture and Biotechnology, 4(3).
  17. Kildisheva, O. A., Dixon, K. W., Silveira, F. A. O., Chapman, T., Di Sacco, A., Mondoni, A., Turner, S. R., & Cross, A. T. (2020). Dormancy and germination: making every seed count in restoration. Restoration Ecology.
  18. Kumar, R., Barman, A., Phukan, T., Kabyashree, K., Singh, N., Jha, G., Sonti, R. V, Genin, S., & Ray, S. K. (2017). Ralstonia solanacearum virulence in tomato seedlings inoculated by leaf clipping. Plant Pathology, 66(5), 835–841.
  19. Leifert, C., & Cassells, A. C. (2001). Microbial hazards in plant tissue and cell cultures. In Vitro Cellular & Developmental Biology-Plant, 37(2), 133–138.
  20. Liu, Q., Ravanlou, A., & Babadoost, M. (2016). Occurrence of bacterial spot on pumpkin and squash fruit in the North Central Region of the United States and bacteria associated with the spots. Plant Disease, 100(12), 2377–2382.
  21. Ndeddy Aka, R. J., & Babalola, O. O. (2016). Effect of bacterial inoculation of strains of Pseudomonas aeruginosa, Alcaligenes feacalis and Bacillus subtilis on germination, growth and heavy metal (Cd, Cr, and Ni) uptake of Brassica juncea. International Journal of Phytoremediation, 18(2), 200–209.
  22. Poorter, H., Fiorani, F., Pieruschka, R., Wojciechowski, T., van der Putten, W. H., Kleyer, M., Schurr, U., & Postma, J. (2016). Pampered inside, pestered outside? Differences and similarities between plants growing in controlled conditions and in the field. New Phytologist, 212(4), 838–855.
  23. Powell, A. (2009). What is seed quality and how to measure it. Responding to the Challenges of a Changing World: The Role of New Plant Varieties and High Quality Seed in Agriculture. Proceedings of the Second World Seed Conference, Rome, 8–10.
  24. Rahman, H. (2016). Investigation on seed health status of cucurbits for storage management.
  25. Ravanlou, A., & Babadoost, M. (2015). Development of bacterial spot, incited by Xanthomonas cucurbitae, in pumpkin fields. HortScience, 50(5), 714–720.
  26. Ruttanaruangboworn, A., Chanprasert, W., Tobunluepop, P., & Onwimol, D. (2017). Effect of seed priming with different concentrations of potassium nitrate on the pattern of seed imbibition and germination of rice (Oryza sativa). Journal of Integrative Agriculture, 16(3), 605–613.
  27. Sharma, A., Katoch, V., & Rana, C. (2016). Important Diseases of Cucurbitaceous Crops and Their Management. 18
  28. Sharma, A., Shukla, A. & Gupta, M. (2023). Effect of bioagents on cucumber seed mycoflora, seed germination, and seedling vigour. Sci Rep. 13(1), 6052.
  29. Sharma, D. K. (2018). Seed-borne and post-harvest diseases of watermelon (Citrullus lanatus (Thunb.) Matsum. Nakai) and their management. Seed.
  30. Souza, R. de, Ambrosini, A., & Passaglia, L. M. P. (2015). Plant growth-promoting bacteria as inoculants in agricultural soils. Genetics and Molecular Biology, 38(4), 401–419.
  31. Sui, Y.-S., Wan, G.-H., Chen, Y.-W., Ku, H.-L., Li, L.-P., Liu, C.-H., & Mau, H.-S. (2012). Effectiveness of bacterial disinfectants on surfaces of mechanical ventilator systems. Respiratory Care, 57(2), 250–256.
  32. Thomas, P., Kumari, S., Swarna, G. K., & Gowda, T. K. S. (2007). Papaya shoot tip associated endophytic bacteria isolated from in vitro cultures and host–endophyte interaction in vitro and in vivo. Canadian Journal of Microbiology, 53(3), 380–390.
  33. Tumpa, F. H., Alam, M. Z., Hossen, K., & Khokon, M. A. R. (2018). Chitosan and yeast elicitor in suppressing seed-borne fungi of cucurbitaceous vegetables. Journal of the Bangladesh Agricultural University, 16(2), 187–192.
  34. Vejsadova, H., Latalova, K., & Řizková, R. (2002). Influence of growth regulators on terrestrial orchid culture under in vitro Acta Pruhoniciana, 73, 27–36.
  35. Wang, P. (2021). Cucumber Economic Values and Its Cultivation and Breeding. Chinese Academy of Agricultural Sciences, 2021, 228.
  36. Zaller, J. G., König, N., Tiefenbacher, A., Muraoka, Y., Querner, P., Ratzenböck, A., Bonkowski, M., & Koller, R. (2016). Pesticide seed dressings can affect the activity of various soil organisms and reduce decomposition of plant material. BMC Ecology, 16(1), 37.