IMPACT OF SPIRULINA PLATENSIS AND STEVIA REBAUDIANA ON GROWTH AND ESSENTIAL OIL PRODUCTION OF BASIL OCIMUM CITRIODORUM
IMPACT OF SPIRULINA PLATENSIS AND STEVIA REBAUDIANA ON GROWTH AND ESSENTIAL OIL PRODUCTION OF BASIL OCIMUM CITRIODORUM
Sherif S. Saleh 1,2* and Nahed S.A. El-Shayeb2
1 Tissue Culture and Nanotechnology Lab., Hort., Res., Institute, A. R. C., Egypt
2 Medicinal and Aromatic Department, Hort., Res., Institute, A. R. C., Egypt
| A R T I C L E I N F O
Article Type: Research Received: 20, Mar. 2020. Accepted: 04, Apr. 2020. Published: 04, Apr. 2020.
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A B S T R A C T
The constituents of essential oils isolated by diethyl ether of the in vitro explants of Ocimum citriodorum cultured on alternative media containing green algae spirulina and stevia plant powder or filtrate were examined by GC-MS. The Spirulina platensis green algae were added in culture medium powder at (0.5, 1.0 and 2.0 g/l) and powder filtrated at 2, 4 and 6 ml/l from concentrations 5, 10 and 15 %, respectively. Also, Stevia rebaudiana dry leaves were added powder at 0.5, 1.0 and 1.5 g/l and powder filtrated 5, 10 and 15 ml/l from concentrations 0.05, 0.1 and 0.15 %, respectively. FTIR and XRD were examined for both materials using as alternatively medium. The explants that were cultured on media containing spirulina filtrate at 4 ml/l and 6 ml/l scored survival 100 % and shootlets length 0.84 and 0.92 cm; shootlet number 1.98 and 2.33; leaves number 8.66 and 2.60, respectively. The explants cultured on media containing stevia filtrate 5 and 10 ml/l also gave the best results but the media turned to browning (++) and (+++), respectively. A total of 12 components were identified accounting for 94.52 % of the oils of O. citriodorum. The oil contained, as main components, geranial (31.24 %), Linalool (8.29 %), Estragole (4.98 %), neral (25.71%) and Neryl acetate (8.857 %). |
| Keywords:
Ocimum citriodorum, stevia rebaudiana, spirulina platensis, GC, MS, Essential oil, in vitro culture, culture media. |
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